.jpg)
Western blot analysis of extracts from normal (control) and CTCF knockout (KO) 293T cells, using CTCF antibody (A19588) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
.jpg)
Western blot analysis of extracts of various cell lines, using CTCF antibody (A19588) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
.jpg)
Immunofluorescence analysis of C6 cells using CTCF antibody (A19588). Blue: DAPI for nuclear staining.
.jpg)
Immunofluorescence analysis of NIH-3T3 cells using CTCF antibody (A19588). Blue: DAPI for nuclear staining.
.jpg)
Chromatin immunoprecipitation analysis of extracts of 293T cells, using CTCF antibody (A19588) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
[KO Validated] CTCF Rabbit mAb
Catalog No：AB-A19588
Manufacturer：ABclonal
Synonyms：CTCF;MRD21;CTCF
Applications：WB,IF,ChIP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] CTCF Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
| Immunogen: | A synthesized peptide derived from human CTCF. |
| Gene ID: | 10664 |
| Swiss prot: | P49711 |
| Molecular weight: | 140kDa |
Help & Support
Product
Guarantee and
Expert Support
Related products
Catalog No：AB-A0994
Manufacturer：ABclonal
Synonyms：HK2;HKII;HXK2;hexokinase-2
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
Catalog No：AB-A0746
Manufacturer：ABclonal
Synonyms：CCND3;cyclin D3;Cyclin D3
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €485,00
Catalog No：AB-A0719
Manufacturer：ABclonal
Synonyms：HMG-1;HMG1;HMG3;SBP-1;HMGB1
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €605,00
Catalog No：AB-A0533
Manufacturer：ABclonal
Synonyms：HK, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, hexokinase
Applications：WB,IHC,IF,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €605,00
Catalog No：AB-A0432
Manufacturer：ABclonal
Synonyms：CD325;CDHN;CDw325;NCAD;N Cadherin;CDH2
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
Catalog No：AB-A0281
Manufacturer：ABclonal
Synonyms：CASP9;APAF-3;APAF3;ICE-LAP6;MCH6;PPP1R56;caspase-9;casp9;Caspase 9
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00