.jpg)
Western blot analysis of extracts of various cell lines, using CRKL Antibody (A11735) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Western blot analysis of extracts from normal (control) and CRKL knockout (KO) HeLa cells, using CRKL antibody (A11735) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
[KO Validated] CRKL Rabbit pAb
Catalog No：AB-A11735
Manufacturer：ABclonal
Synonyms：CRKL
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] CRKL Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human CRKL (NP_005198.1). |
| Gene ID: | 1399 |
| Swiss prot: | P46109 |
| Molecular weight: | 33kDa |
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