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Western blot analysis of extracts from normal (control) and CDK2AP1 knockout (KO) 293T cells, using CDK2AP1 antibody (A19985) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
[KO Validated] CDK2AP1 Rabbit pAb
Catalog No：AB-A19985
Manufacturer：ABclonal
Synonyms：CDK2AP1;DOC1;DORC1;ST19;doc-1;p12DOC-1
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] CDK2AP1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Immunogen: | Recombinant protein of human CDK2AP1. |
| Gene ID: | 8099 |
| Swiss prot: | O14519 |
| Molecular weight: | 9kDa/12kDa |
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