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Western blot analysis of extracts from normal (control) and Calpain 1 knockout (KO) HeLa cells, using Calpain 1 antibody (A18006) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
[KO Validated] Calpain 1 Rabbit pAb
Catalog No：AB-A18006
Manufacturer：ABclonal
Synonyms：CAPN1;CANP;CANP1;CANPL1;SPG76;muCANP;muCL;calpain 1
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] Calpain 1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human Calpain 1 (NP_005177.2). |
| Gene ID: | 823 |
| Swiss prot: | P07384 |
| Molecular weight: | 81kDa |
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