Western blot analysis of extracts from normal (control) and BUB1 knockout (KO) HeLa cells, using BUB1 antibody (A18053) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1min.
[KO Validated] BUB1 Rabbit pAb
Catalog No:AB-A18053
Manufacturer:ABclonal
Synonyms:BUB1;BUB1A;BUB1L;hBUB1
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] BUB1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BUB1 (NP_004327.1). |
Gene ID: | 699 |
Swiss prot: | O43683 |
Molecular weight: | 115kDa/120kDa/122kDa |
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