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Western blot analysis of extracts of various cell lines, using BRG1/BRG1/SMARCA4 antibody (A0887) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.
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Western blot analysis of extracts from normal (control) and BRG1/BRG1/SMARCA4 knockout (KO) 293T cells, using BRG1/BRG1/SMARCA4 antibody (A0887) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human colon using BRG1/BRG1/SMARCA4 antibody (A0887) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using BRG1/BRG1/SMARCA4 antibody (A0887) at dilution of 1:100 (40x lens).
[KO Validated] BRG1/SMARCA4 Rabbit pAb
Catalog No：AB-A0887
Manufacturer：ABclonal
Synonyms：BAF190;BAF190A;BRG1;CSS4;MRD16;RTPS2;SNF2;SNF2L4;SNF2LB;SWI2;hSNF2b;SMARCA4
Applications：WB,IHC,IF
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] BRG1/SMARCA4 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 30-130 of human BRG1/BRG1/SMARCA4 (NP_003063.2). |
| Gene ID: | 6597 |
| Swiss prot: | P51532 |
| Molecular weight: | 181kDa/184kDa |
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