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Western blot analysis of extracts from normal (control) and BRD4 Rabbit pAb knockout (KO) HeLa cells, using BRD4 Rabbit pAb antibody (A20019) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
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Western blot analysis of extracts of HeLa cells, using BRD4 antibody (A20019) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
[KO Validated] BRD4 Rabbit pAb
Catalog No：AB-A20019
Manufacturer：ABclonal
Synonyms：BRD4;CAP;HUNK1;HUNKI;MCAP;BRD4 short isoform
Applications：WB,IHC
Reactivity：Human,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] BRD4 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. |
| Immunogen: | Recombinant protein of human BRD4 |
| Gene ID: | 23476 |
| Swiss prot: | O60885-2 |
| Molecular weight: | 80kDa/88kDa/152kDa |
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