Western blot analysis of extracts of various cell lines, using Beclin 1 antibody (A7353) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Western blot analysis of extracts from normal (control) and Beclin 1 knockout (KO) 293T cells, using Beclin 1 antibody (A7353) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
[KO Validated] Beclin 1 Rabbit pAb
Catalog No:AB-A7353
Manufacturer:ABclonal
Synonyms:ATG6;VPS30;beclin1;Beclin 1;BECN1;beclin-1
Applications:WB,IHC,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | [KO Validated] Beclin 1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1). |
Gene ID: | 8678 |
Swiss prot: | Q14457 |
Molecular weight: | 51kDa |
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