Western blot analysis of extracts from normal (control) and Bax knockout (KO) 293T cells, using Bax antibody (A20227) at 1:2000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Western blot analysis of extracts of HT-1080 cells, using Bax antibody (A20227) at 1:2000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
[KO Validated] Bax Rabbit mAb
Catalog No:AB-A20227
Manufacturer:ABclonal
Synonyms:BCL2L4;BAX
Applications:WB,IHC,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] Bax Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. |
Immunogen: | A synthetic peptide of human Bax. |
Gene ID: | 581 |
Swiss prot: | Q07812 |
Molecular weight: | 4kDa/12kDa/15kDa/18kDa/19kDa/21kDa/24kDa |
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