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Western blot analysis of extracts from normal (control) and ATG4D knockout (KO) HeLa cells, using ATG4D antibody (A18097) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
[KO Validated] ATG4D Rabbit pAb
Catalog No：AB-A18097
Manufacturer：ABclonal
Synonyms：ATG4D;APG4-D;APG4D;AUTL4
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] ATG4D Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ATG4D (NP_116274.3). |
| Gene ID: | 84971 |
| Swiss prot: | Q86TL0 |
| Molecular weight: | 16kDa/52kDa |
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