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Western blot analysis of extracts of various cell lines, using ARHGEF1 antibody (A4274) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Western blot analysis of extracts from normal (control) and ARHGEF1 knockout (KO) 293T cells, using ARHGEF1 antibody (A4274) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded human tonsil using ARHGEF1 antibody (A4274) at dilution of 1:100 (40x lens).
[KO Validated] ARHGEF1 Rabbit pAb
Catalog No：AB-A4274
Manufacturer：ABclonal
Synonyms：ARHGEF1;GEF1;LBCL2;LSC;P115-RHOGEF;SUB1.5
Applications：WB,IHC,IF
Reactivity：Human,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] ARHGEF1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 783-912 of human ARHGEF1 (NP_004697.2). |
| Gene ID: | 9138 |
| Swiss prot: | Q92888 |
| Molecular weight: | 98kDa/102kDa/104kDa/105kDa |
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