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Western blot analysis of extracts of various cell lines, using APEX1/APE1 antibody (A2587).|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.
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Western blot analysis of extracts from normal (control) and APEX1/APE1 knockout (KO) 293T cells, using APEX1/APE1 antibody (A2587) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
[KO Validated] APEX1/APE1 Rabbit pAb
Catalog No：AB-A2587
Manufacturer：ABclonal
Synonyms：APEX1;APE;APE1;APEN;APEX;APX;HAP1;REF1
Applications：WB,IF,IP
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] APEX1/APE1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5′ to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1/APE1 (NP_542380.1). |
| Gene ID: | 328 |
| Swiss prot: | P27695 |
| Molecular weight: | 35kDa |
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