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Western blot analysis of extracts of various cell lines, using AMPKβ1 antibody (A12491) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Western blot analysis of extracts from normal (control) and AMPKβ1 knockout (KO) HeLa cells, using AMPKβ1 antibody (A12491) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded human esophagus using AMPKβ1 antibody (A12491) at dilution of 1:100 (40x lens).
[KO Validated] AMPKβ1 Rabbit pAb
Catalog No：AB-A12491
Manufacturer：ABclonal
Synonyms：PRKAB1;AMPK;HAMPKb
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] AMPKβ1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human AMPKβ1 (NP_006244.2). |
| Gene ID: | 5564 |
| Swiss prot: | Q9Y478 |
| Molecular weight: | 30kDa |
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