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Western blot analysis of extracts of various cell lines, using AMPKα2 antibody (A7339) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 20s.
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Western blot analysis of extracts from normal (control) and AMPKα2 Rabbit pAb knockout (KO) HCT116 cells, using AMPKα2 Rabbit pAb antibody (A7339) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
[KO Validated] AMPKα2 Rabbit pAb
Catalog No：AB-A7339
Manufacturer：ABclonal
Synonyms：PRKAA2;AMPK;AMPK2;AMPKa2;PRKAA
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] AMPK伪2 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 343-552 of human AMPK伪2 (NP_006243.2). |
| Gene ID: | 5563 |
| Swiss prot: | P54646 |
| Molecular weight: | 62kDa |
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