Western blot analysis of extracts of various cell lines, using AKT2 antibody (A18019) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Western blot analysis of extracts from normal (control) and AKT2 knockout (KO) 293T cells, using AKT2 antibody (A18019) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
[KO Validated] AKT2 Rabbit pAb
Catalog No:AB-A18019
Manufacturer:ABclonal
Synonyms:AKT2;HIHGHH;PKBB;PKBBETA;PRKBB;RAC-BETA
Applications:WB,IHC,ICC,IF,IP,ChIP
Reactivity:Human,Mouse,Rat,Monkey
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] AKT2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. |
Immunogen: | Recombinant protein of human AKT2 |
Gene ID: | 208 |
Swiss prot: | P31751 |
Molecular weight: | 51kDa/55kDa |
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