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Western blot analysis of extracts from normal (control) and 14-3-3 gamma knockout (KO) 293T cells, using 14-3-3 gamma antibody (A18060) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
[KO Validated] 14-3-3 gamma Rabbit pAb
Catalog No：AB-A18060
Manufacturer：ABclonal
Synonyms：YWHAG;14-3-3GAMMA;PPP1R170
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] 14-3-3 gamma Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. |
| Immunogen: | A synthetic peptide of human 14-3-3 gamma |
| Gene ID: | 7532 |
| Swiss prot: | P61981 |
| Molecular weight: | 28kDa |
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