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Western blot analysis of extracts of various cell lines, using α-Catenin antibody (A5635) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
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Western blot analysis of extracts from normal (control) and α-Catenin Rabbit pAb knockout (KO) 293T cells, using α-Catenin Rabbit pAb antibody (A5635) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat kidney using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human vermiform appendix using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).
[KO Validated] α-Catenin Rabbit pAb
Catalog No：AB-A5635
Manufacturer：ABclonal
Synonyms：CAP102;MDPT2;Catenin alpha 1;CTNNA1
Applications：WB,IHC,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] 伪-Catenin Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 637-906 of human 伪-Catenin (NP_001894.2). |
| Gene ID: | 1495 |
| Swiss prot: | P35221 |
| Molecular weight: | 59kDa/100kDa/102kDa |
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