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Western blot analysis of extracts of various cell lines, using KIF14 antibody (A10275) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
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Immunohistochemistry of paraffin-embedded rat ovary using KIF14 antibody (A10275) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using KIF14 antibody (A10275) at dilution of 1:100 (40x lens).
KIF14 Rabbit pAb
Catalog No：AB-A10275
Manufacturer：ABclonal
Synonyms：KIF14;MKS12
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | KIF14 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the kinesin-3 superfamily of microtubule motor proteins. These proteins are involved in numerous processes including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. In human HeLa-S3 and 293T cells, this protein is localized to the cytoplasm during interphase, to the spindle poles and spindle microtubules during mitosis, and to the midbody during cytokinesis. An internal motor domain displays microtubule-dependent ATPase activity, consistent with its function as a microtubule motor protein. Knockdown of this gene results in failed cytokinesis with endoreplication, which results in multinucleated cells. This gene has been identified as a likely oncogene in breast, lung and ovarian cancers, as well as retinoblastomas and gliomas. Alternative splicing results in multiple transcript variants. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1399-1648 of human KIF14 (NP_055690.1). |
| Gene ID: | 9928 |
| Swiss prot: | Q15058 |
| Molecular weight: | 186kDa |
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