Western blot analysis of extracts of HeLa cells, using JNK1/2 antibody (A18287) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using JNK1/2 antibody (A18287) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
JNK1/2 Rabbit pAb
Catalog No:AB-A18287
Manufacturer:ABclonal
Synonyms:JNK1/2
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | JNK1/2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | JNK belongs to MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation.JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1,JNK2,and JNK3 |
Immunogen: | Recombinant protein of human JNK1/2. |
Gene ID: | 5599/5601 |
Swiss prot: | P45983/P45984 |
Molecular weight: |
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