Western blot analysis of extracts of various cell lines, using IQGAP1 antibody (A5679) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
IQGAP1 Rabbit pAb
Catalog No:AB-A5679
Manufacturer:ABclonal
Synonyms:IQGAP1;HUMORFA01;SAR1;p195
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | IQGAP1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-750 of human IQGAP1 (NP_003861.1). |
Gene ID: | 8826 |
Swiss prot: | P46940 |
Molecular weight: | 189kDa |
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