Western blot analysis of extracts of various cell lines, using INSIG2 antibody (A1992) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 1s.
INSIG2 Rabbit pAb
Catalog No:AB-A1992
Manufacturer:ABclonal
Synonyms:INSIG2;INSIG-2
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | INSIG2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human INSIG2 (NP_057217.2). |
Gene ID: | 51141 |
Swiss prot: | Q9Y5U4 |
Molecular weight: | 24kDa |
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