Western blot analysis of extracts of various cell lines, using INCENP antibody (A0622) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
INCENP Rabbit pAb
Catalog No:AB-A0622
Manufacturer:ABclonal
Synonyms:INCENP
Applications:WB
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | INCENP Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM, Mar 2008] |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 785-914 of human INCENP (NP_064623.2). |
Gene ID: | 3619 |
Swiss prot: | Q9NQS7 |
Molecular weight: | 104kDa/105kDa |
Help & Support
Product
Guarantee and
Expert Support
Related products
Catalog No:AB-A0109
Manufacturer:ABclonal
Synonyms:D3F15S2, DNF15S2, HGFL, MSP, NF15S2
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Catalog No:AB-A0106
Manufacturer:ABclonal
Synonyms:MCPH12, PLSTIRE
Applications:WB
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Catalog No:AB-A0094
Manufacturer:ABclonal
Synonyms:CDKN2, p33(CDK2)
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Catalog No:AB-A0102
Manufacturer:ABclonal
Synonyms:FOXO3;AF6q21;FKHRL1;FKHRL1P2;FOXO2;FOXO3A
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Catalog No:AB-A0036
Manufacturer:ABclonal
Synonyms:HEL-S-94n, HOP, IEF-SSP-3521, P60, STI1, STI1L
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Catalog No:AB-A0027
Manufacturer:ABclonal
Synonyms:CANDF7;IMD31A;IMD31B;IMD31C;ISGF-3;STAT91;STAT1 alpha;STAT1
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00