Western blot analysis of extracts of various cell lines, using IL5RA antibody (A8552) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
IL5RA Rabbit pAb
Catalog No:AB-A8552
Manufacturer:ABclonal
Synonyms:IL5RA;CD125;CDw125;HSIL5R3;IL5R
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | IL5RA Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-335 of human IL5RA (NP_783855.1). |
Gene ID: | 3568 |
Swiss prot: | Q01344 |
Molecular weight: | 23kDa/37kDa/42kDa/47kDa |
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