Western blot analysis of extracts of various cell lines, using IL5 antibody (A7690) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 15s.
IL5 Rabbit pAb
Catalog No:AB-A7690
Manufacturer:ABclonal
Synonyms:IL5;EDF;IL-5;TRF
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | IL5 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-134 of human IL5 (NP_000870.1). |
Gene ID: | 3567 |
Swiss prot: | P05113 |
Molecular weight: | 15kDa |
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