Western blot analysis of extracts of various cell lines, using IL1β antibody (A11370) at 1:1000 dilution. THP-1 cells were treated by PMA (80 nM) for overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
IL1β Rabbit pAb
Catalog No:AB-A11370
Manufacturer:ABclonal
Synonyms:IL-1;IL1-BETA;IL1F2;IL1 beta;IL1B
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | IL1β Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 124-223 of human IL1β (NP_000567.1). |
Gene ID: | 3553 |
Swiss prot: | P01584 |
Molecular weight: | 30kDa |
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