Western blot analysis of extracts of various cell lines, using IL17A antibody (A12454) at 1:1000 dilution.Jurkat cells were treated by TPA (40 nM),A23187 (2μM) and Brefeldin A (300 ng / ml) for 0-24hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Western blot analysis of extracts of Mouse thymus, using IL17A antibody (A12454) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Western blot analysis of extracts of Recombinant Human IL-17A Protein, using IL17A antibody (A12454) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
IL17A Rabbit pAb
Catalog No:AB-A12454
Manufacturer:ABclonal
Synonyms:IL17A;CTLA-8;CTLA8;IL-17;IL-17A;IL17
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | IL17A Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-155 of human IL17A (NP_002181.1). |
Gene ID: | 3605 |
Swiss prot: | Q16552 |
Molecular weight: | 17kDa |
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