Western blot analysis of extracts of various cell lines, using IL12B antibody (A13024) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 20s.
IL12B Rabbit pAb
Catalog No:AB-A13024
Manufacturer:ABclonal
Synonyms:IL12B;CLMF;CLMF2;IL-12B;IMD28;IMD29;NKSF;NKSF2
Applications:WB,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | IL12B Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-328 of human IL12B (NP_002178.2). |
Gene ID: | 3593 |
Swiss prot: | P29460 |
Molecular weight: | 37kDa |
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