Western blot analysis of extracts of various cell lines, using IL10 antibody (A12255) at 1:1000 dilution.THP-1 cells were treated by LPS and Brefeldin A for different times of stimulation.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Western blot analysis of extracts of Rat thymus, using IL10 antibody (A12255) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
IL10 Rabbit mAb
Catalog No:AB-A12255
Manufacturer:ABclonal
Synonyms:CSIF, GVHDS, IL-10, IL10A, TGIF
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | IL10 Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011] |
Immunogen: | A synthesized peptide derived from human IL10 |
Gene ID: | 3586 |
Swiss prot: | P22301 |
Molecular weight: | 18kDa |
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