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Western blot analysis of extracts of various cell lines, using IL1β antibody (A16288) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Western blot analysis of extracts of THP-1 cells, using IL1β antibody (A16288) at 1:1000 dilution.THP-1 cells were treated by LPS (1 μg/mL) at 37℃ for 6 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat lung using IL1β antibody (A16288) at dilution of 1:100 (40x lens).
IL1β Rabbit pAb
Catalog No：AB-A16288
Manufacturer：ABclonal
Synonyms：IL-1;IL1-BETA;IL1F2;IL1 beta;IL1B
Applications：WB,IHC,IF,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | IL1β Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. |
| Immunogen: | A synthetic peptide of human IL1β |
| Gene ID: | 3553 |
| Swiss prot: | P01584 |
| Molecular weight: | 30kDa |
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