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Western blot analysis of extracts of various cell lines, using IDH3A antibody (A14650) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat kidney using IDH3A antibody (A14650) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human thyroid cancer using IDH3A antibody (A14650) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using IDH3A antibody (A14650) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using IDH3A antibody (A14650) at dilution of 1:100 (40x lens).
IDH3A Rabbit pAb
Catalog No：AB-A14650
Manufacturer：ABclonal
Synonyms：IDH3A
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | IDH3A Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 28-366 of human IDH3A (NP_005521.1). |
| Gene ID: | 3419 |
| Swiss prot: | P50213 |
| Molecular weight: | 31kDa/40kDa |
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