Western blot analysis of extracts of A-549 cells, using ICAM-1/CD54 antibody (A5597) at 1:1000 dilution.A-549 cells were treated by IFN-α (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.A-549 cells were treated by IFNG (100 ng/mL) at 37℃ for 48 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
ICAM-1/CD54 Rabbit pAb
Catalog No:AB-A5597
Manufacturer:ABclonal
Synonyms:BB2;CD54;P3.58;ICAM1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | ICAM-1/CD54 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-480 of human ICAM-1/CD54 (NP_000192.2). |
Gene ID: | 3383 |
Swiss prot: | P05362 |
Molecular weight: | 57kDa |
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