Western blot analysis of extracts of THP-1 cells, using Iba1 Rabbit pAb (A1527) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
Western blot analysis of extracts of various cell lines, using Iba1 Rabbit pAb (A1527) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 60s.
Western blot analysis of extracts of Mouse spleen, using Iba1 Rabbit pAb (A1527) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Iba1 Rabbit pAb
Catalog No:AB-A1527
Manufacturer:ABclonal
Synonyms:AIF1;AIF-1;IBA1;IRT-1;IRT1;Iba1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Iba1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human Iba1 (NP_001614.3). |
Gene ID: | 199 |
Swiss prot: | P55008 |
Molecular weight: | 10kDa/14kDa/16kDa |
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