Western blot analysis of extracts of A-549 cells, using HLA-F antibody (A10384) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
HLA-F Rabbit pAb
Catalog No:AB-A10384
Manufacturer:ABclonal
Synonyms:HLA-F;CDA12;HLA-5.4;HLA-CDA12;HLAF
Applications:WB
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | HLA-F Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-190 of human HLA-F (NP_001091949.1). |
Gene ID: | 3134 |
Swiss prot: | P30511 |
Molecular weight: | 28kDa/39kDa/50kDa |
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