Western blot analysis of extracts of Raji cells, using HLA-DQB2 antibody (A11605) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
HLA-DQB2 Rabbit pAb
Catalog No:AB-A11605
Manufacturer:ABclonal
Synonyms:HLA-DQB2;HLA-DQB1;HLA-DXB;major histocompatibility complex;class II;DQ beta 2
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | HLA-DQB2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | HLA-DQB2 belongs to the family of HLA class II beta chain paralogs. Class II molecules are heterodimers consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. They play a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). Polymorphisms in the alpha and beta chains specify the peptide binding specificity, and typing for these polymorphisms is routinely done for bone marrow transplantation. However this gene, HLA-DQB2, is not routinely typed, as it is not thought to have an effect on transplantation. There is conflicting evidence in the literature and public sequence databases for the protein-coding capacity of HLA-DQB2. Because there is evidence of transcription and an intact ORF, HLA-DQB2 is represented in Entrez Gene and in RefSeq as a protein-coding locus. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 28-227 of human HLA-DQB2 (NP_001185787.1). |
Gene ID: | 3120 |
Swiss prot: | P05538 |
Molecular weight: | 26kDa/30kDa |
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