Western blot analysis of extracts of various cell lines, using HLA-DPB1 antibody (A13312) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
HLA-DPB1 Rabbit pAb
Catalog No:AB-A13312
Manufacturer:ABclonal
Synonyms:DPB1;HLA-DP;HLA-DP1B;HLA-DPB;HLA-DPB1;major histocompatibility complex;class II;DP beta 1
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | HLA-DPB1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-225 of human HLA-DPB1 (NP_002112.3). |
Gene ID: | 3115 |
Swiss prot: | P04440 |
Molecular weight: | 29kDa |
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