Western blot analysis of extracts of various cell lines, using HLA-DOB antibody (A12152) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
HLA-DOB Rabbit pAb
Catalog No:AB-A12152
Manufacturer:ABclonal
Synonyms:HLA-DOB;DOB;HLA_DOB;major histocompatibility complex;class II;DO beta
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | HLA-DOB Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 27-224 of human HLA-DOB (NP_002111.1). |
Gene ID: | 3112 |
Swiss prot: | P13765 |
Molecular weight: | 30kDa |
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Applications:WB,IHC,IF
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Conjugate:No
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Conjugate:No
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Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
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Applications:IF
Reactivity:Human
Host:Rabbit
Conjugate:No
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Manufacturer:ABclonal
Synonyms:TLR4;ARMD10;CD284;TLR-4;TOLL
Applications:WB,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
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