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Western blot analysis of extracts of various cell lines, using Histone H4 antibody (A1131) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat brain using Histone H4 antibody (A1131) at dilution of 1:200 (40x lens).
Histone H4 Rabbit pAb
Catalog No：AB-A1131
Manufacturer：ABclonal
Synonyms：H4FA;Histone H4;HIST1H4A;HIST4H4;H4/p
Applications：WB,IHC
Reactivity：Human,Mouse,Rat,Other (Wide Range)
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Histone H4 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-103 of human Histone H4 (NP_001029249.1). |
| Gene ID: | 8359 |
| Swiss prot: | P62805 |
| Molecular weight: | 11kDa |
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