Western blot analysis of extracts of various cell lines, using HIF1β/ARNT antibody (A0972) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
HIF1β/ARNT Rabbit pAb
Catalog No:AB-A0972
Manufacturer:ABclonal
Synonyms:ARNT;HIF-1-beta;HIF-1beta;HIF1-beta;HIF1B;HIF1BETA;TANGO;bHLHe2
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | HIF1β/ARNT Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 400-500 of human HIF1β/ARNT (NP_001659.1). |
Gene ID: | 405 |
Swiss prot: | P27540 |
Molecular weight: | 84kDa/85kDa/86kDa |
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