Western blot analysis of extracts of various cell lines, using HEC1/HEC1/NDC80 antibody (A5411) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
HEC1/NDC80 Rabbit pAb
Catalog No:AB-A5411
Manufacturer:ABclonal
Synonyms:NDC80;HEC;HEC1;HsHec1;KNTC2;TID3;hsNDC80
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | HEC1/NDC80 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 343-642 of human HEC1/HEC1/NDC80 (NP_006092.1). |
Gene ID: | 10403 |
Swiss prot: | O14777 |
Molecular weight: | 73kDa |
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