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Western blot analysis of extracts of various cell lines, using HDAC4 antibody (A12552) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
HDAC4 Rabbit pAb
Catalog No：AB-A12552
Manufacturer：ABclonal
Synonyms：HDAC4;AHO3;BDMR;HA6116;HD4;HDAC-4;HDAC-A;HDACA
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | HDAC4 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 451-650 of human HDAC4 (NP_006028.2). |
| Gene ID: | 9759 |
| Swiss prot: | P56524 |
| Molecular weight: | 106kDa/119kDa |
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