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Western blot analysis of extracts of various cell lines, using HDAC3 antibody (A12542) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
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Western blot analysis of extracts of mouse brain, using HDAC3 antibody (A12542) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded mouse liver using HDAC3 antibody (A12542) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using HDAC3 antibody (A12542) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using HDAC3 antibody (A12542) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using HDAC3 antibody (A12542) at dilution of 1:100 (40x lens).
HDAC3 Rabbit pAb
Catalog No：AB-A12542
Manufacturer：ABclonal
Synonyms：HD3;RPD3;RPD3-2;HDAC3
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | HDAC3 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 330 to the C-terminus of human HDAC3 (NP_003874.2). |
| Gene ID: | 8841 |
| Swiss prot: | O15379 |
| Molecular weight: | 48kDa/49kDa |
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