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Western blot analysis of extracts of various cell lines, using HDAC3 antibody (A19537) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1min.
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Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug HDAC3 antibody (A19537). Western blot was performed from the immunoprecipitate using HDAC3 (A19537) at a dilition of 1:1000.
HDAC3 Rabbit mAb
Catalog No：AB-A19537
Manufacturer：ABclonal
Synonyms：HD3;RPD3;RPD3-2;HDAC3
Applications：WB,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | HDAC3 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human HDAC3. |
| Gene ID: | 8841 |
| Swiss prot: | O15379 |
| Molecular weight: | 49kDa |
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