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Western blot analysis of extracts of RAW264.7 cells, using GSDMD antibody (A17308) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
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Western blot analysis of extracts of various cell lines, using GSDMD antibody (A17308) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
GSDMD (Full Length+C terminal) Rabbit pAb
Catalog No：AB-A17308
Manufacturer：ABclonal
Synonyms：GSDMD;DF5L;DFNA5L;FKSG10;GSDMDC1
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | GSDMD (Full Length+C terminal) Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 276-484 of human GSDMD (NP_079012.3). |
| Gene ID: | 79792 |
| Swiss prot: | P57764 |
| Molecular weight: | 53kDa |
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