Western blot analysis of extracts of various cell lines, using Glypican 3 (Glypican 3 (GPC3)) antibody (A12384) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Glypican 3 (GPC3) Rabbit pAb
Catalog No:AB-A12384
Manufacturer:ABclonal
Synonyms:GPC3;DGSX;GTR2-2;MXR7;OCI-5;SDYS;SGB;SGBS;SGBS1;glypican-3
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Glypican 3 (GPC3) Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 290-550 of human Glypican 3 (Glypican 3 (GPC3)) (NP_004475.1). |
Gene ID: | 2719 |
Swiss prot: | P51654 |
Molecular weight: | 59kDa/65kDa/68kDa |
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