Western blot analysis of extracts of various cell lines, using Glutaminase (Glutaminase (GLS)) antibody (A3885) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Glutaminase (GLS) Rabbit pAb
Catalog No:AB-A3885
Manufacturer:ABclonal
Synonyms:GLS;AAD20;GAC;GAM;GLS1;KGA
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Glutaminase (GLS) Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 610-669 of human Glutaminase (Glutaminase (GLS)) (NP_055720.3). |
Gene ID: | 2744 |
Swiss prot: | O94925 |
Molecular weight: | 17kDa/65kDa/73kDa |
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