Western blot analysis of extracts of various cell lines, using GLUD1 antibody (A7631) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
GLUD1 Rabbit pAb
Catalog No:AB-A7631
Manufacturer:ABclonal
Synonyms:GLUD1;GDH;GDH1;GLUD
Applications:WB,IHC,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | GLUD1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 54-240 of human GLUD1 (NP_005262.1). |
Gene ID: | 2746 |
Swiss prot: | P00367 |
Molecular weight: | 42kDa/46kDa/61kDa |
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