Western blot analysis of extracts of various cell lines, using Glucokinase (Glucokinase (GCK)) antibody (A6293) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Glucokinase (GCK) Rabbit pAb
Catalog No:AB-A6293
Manufacturer:ABclonal
Synonyms:GCK;FGQTL3;GK;GLK;HHF3;HK4;HKIV;HXKP;LGLK;MODY2
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Glucokinase (GCK) Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Glucokinase (Glucokinase (GCK)) (NP_000153.1). |
Gene ID: | 2645 |
Swiss prot: | P35557 |
Molecular weight: | 52kDa |
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