Western blot analysis of extracts of various cell lines, using GLIS3 antibody (A12166) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
GLIS3 Rabbit pAb
Catalog No:AB-A12166
Manufacturer:ABclonal
Synonyms:GLIS3;NDH;ZNF515
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | GLIS3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only two have been determined. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 606-775 of human GLIS3 (NP_689842.3). |
Gene ID: | 169792 |
Swiss prot: | Q8NEA6 |
Molecular weight: | 83kDa/99kDa |
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