Western blot analysis of extracts of various cell lines, using GLI3 Rabbit pAb (A15613) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
GLI3 Rabbit pAb
Catalog No:AB-A15613
Manufacturer:ABclonal
Synonyms:GLI3;ACLS;GCPS;GLI3-190;GLI3FL;PAP-A;PAPA;PAPA1;PAPB;PHS;PPDIV
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | GLI3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 800-900 of human GLI3 (NP_000159.3). |
Gene ID: | 2737 |
Swiss prot: | P10071 |
Molecular weight: | 169kDa |
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