Western blot analysis of extracts of various cell lines, using GEMIN4 antibody (A10764) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
GEMIN4 Rabbit pAb
Catalog No:AB-A10764
Manufacturer:ABclonal
Synonyms:GEMIN4;HC56;HCAP1;HHRF-1;p97
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | GEMIN4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The product of this gene is part of a large complex localized to the cytoplasm, nucleoli, and to discrete nuclear bodies called Gemini bodies (gems). The complex functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. The encoded protein directly interacts with a DEAD box protein and several spliceosome core proteins. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-350 of human GEMIN4 (NP_056536.2). |
Gene ID: | 50628 |
Swiss prot: | P57678 |
Molecular weight: | 120kDa |
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